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RDYH58 functional exosomes targeting myofibroblasts loaded with siFKBP10 for inhibition of collagen biosynthesis and secretion of IPF 期刊论文

编号：

7642323E55C32836A62B999E771EF24F
作者：

Yuan, Ranran#^[1]Mu, Zhen^[1];Zhang, Houqian^[1];Tian, Yu^[1];Xin, Quanlin^[1];Tu, Qingchao^[2,3];Zhang, Yan^[1];Li, Yanqiu(李艳秋)^[4]Zhang, Zhiwen^[5];Chu, Yongchao^[1];Wang, Aiping^[1];Tian, Jingwei^[1];Wang, Hongbo*^[1]Qiu, Chong*^[2,3]Shi, Yanan*^[1]
语种：

英文
期刊：

ACTA PHARMACEUTICA SINICA B ISSN：2211-3835 2025 年 15 卷 12 期 (6681 - 6697) ; DEC
收录：
关键词：

Idiopathic pulmonary fibrosis Myofibroblasts FK506-Binding protein RDYH58 Exosomes Drug delivery Small interfering RNA Ultrasonic microfluidics method
摘要：

Idiopathic pulmonary fibrosis (IPF) is a complex interstitial lung disease in which myofibroblasts are the primary effector cells. FK506-binding protein (FKBP10), a procollagen chaperone, is up-regulated in IPF and primarily localizes to myofibroblasts. Exosomes have garnered significant attention as novel drug delivery vehicles, particularly when engineered. However, myofibroblasts remain underexplored in terms of engineered exosome-based therapies and associated drug targets. In this study, RDYH58, a peptide that targets myofibroblasts, was conjugated to the exosomal membrane protein Lamp2b to produce RDYH58-linked exosomes (RDYH58-exo). In vitro and in vivo experiments demonstrated that compared to unmodified exosomes (unm-exo), RDYH58-exo preferentially localized to myofibroblasts. A small interfering RNA targeting FKBP10 (siFKBP10) was loaded into exosomes using ultrasonic microfluidics method, and the antifibrotic effects of RDYH58-exo carrying siFKBP10 (RDYH58-siFKBP10) were assessed both in vitro and in vivo. The results demonstrated that RDYH58-siFKBP10 effectively silenced FKBP10 gene expression, significantly inhibiting fibroblast activation and extracellular matrix deposition, with superior antifibrotic efficacy compared to unmodified exosome vectors (unm-siFKBP10). RNA-seq analysis confirmed the pivotal regulatory role of FKBP10, providing critical evidence for the development of targeted therapeutic strategies. The RDYH58-siFKBP10 delivery system developed in this study demonstrates remarkable clinical translation potential. (c) 2025 The Authors. Published by Elsevier B.V. on behalf of Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
推荐引用方式
GB/T 7714：

Yuan Ranran,Mu Zhen,Zhang Houqian, et al. RDYH58 functional exosomes targeting myofibroblasts loaded with siFKBP10 for inhibition of collagen biosynthesis and secretion of IPF [J].ACTA PHARMACEUTICA SINICA B,2025,15(12):6681-6697.
APA：

Yuan Ranran,Mu Zhen,Zhang Houqian,Tian Yu,&Shi Yanan.(2025).RDYH58 functional exosomes targeting myofibroblasts loaded with siFKBP10 for inhibition of collagen biosynthesis and secretion of IPF .ACTA PHARMACEUTICA SINICA B,15(12):6681-6697.
MLA：

Yuan Ranran, et al. "RDYH58 functional exosomes targeting myofibroblasts loaded with siFKBP10 for inhibition of collagen biosynthesis and secretion of IPF" .ACTA PHARMACEUTICA SINICA B 15,12(2025):6681-6697.
入库时间：

12/26/2025 10:48:16 AM
更新时间：

3/10/2026 9:51:34 PM
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